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Nature Letter co-authored by CAi and AG Simon

"In vivo FRET-FLIM reveals cell-type-specific protein interactions in Arabidopsis roots"

This Nature Letter focuses on the early development and cell fate in roots of the model plant Arabidopsis thaliana. The separation of cortex and endodermis in the roots is mediated by three co-expressed and interacting transcription factors, named SHORT-ROOT (SHR), SCARECROW (SCR) and JACKDAW (JKD). It has been challenging to get insights how these proteins interact because it was difficult to directly visualize transcription factor complexes. Stefanie Weidtkamp-Peters (CAi), Yvonne Stahl and Rüdiger Simon (Institute of Developmental Genetics & CEPLASand their colleagues from Wageningen, Amsterdam and Madrid were now able to improve the analysis of protein-protein interactions of those transcription factors: They optimized in vivo FRET-FLIM (Förster resonance energy transfer measured by fluorescence lifetime microscopy) at physiological conditions in living Arabidopsis roots. The optimized method enabled them to show how cell-type-specific interaction is established and that cell fate regulators can form higher order complexes.

Furthermore their results indicate that conformational changes or composition of those transcription factor complexes differentially control target genes and thereby specify distinct cell fates.

Full article: undefinedhttps://www.nature.com/nature/journal/v548/n7665/full/nature23317.html

Picture of the month

Click to see image time series

Live-cell Super-Resolution imaging of mitochondrial protein TOM20 labeled with Cell-SNAP-TMR. The image series on the right side was produced by using super-resolution radial fluctuations (SRRF – pronounced as surf). SRRF can be used with widefield and confocal microscopes and is available via the NanoJ-SRRF plugin for ImageJ. Each SRRF frame was produced by running SRRF analysis on 100 frames (10ms each) of the raw TIRF acquisition. These frames were averaged in the left time series for comparison. In contrast to other Super-Resolution techniques SRRF is capable of fast live-cell imaging over long time periods.

For more information please contact us and visit the NanoJ-SRRF Wiki of the R. Henriques-Lab (link):

Images acquired at the Zeiss ELYRA PS1


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