The Olympus Fluoview 1000 confocal scanner with TIRF module is mounted on an inverted IX81 microscope. The microscope is equipped with the PicoQuant FLIM system "HydraHarp400", and a Multiparameter Fluorescence Imaging Spectroscopy (MFIS) extension with six detection channels developed in the Seidel group.
Available laser lines for continuous wave (cw) excitation are: 405 nm, 458 nm, 488 nm, 515 nm, 559 nm and 635 nm. Available laser lines for pulsed and cw excitation, e.g. for FLIM experiments are: 440 nm, 485 nm and 640 nm
In addition to the confocal detection unit, this system also contains a TIRF-module, which can be used with the internal lasers. For detection in TIRF-mode, an Andor EMCCD camera is used.
The system contains an on-stage incubator.
Possible applications for this system are: confocal (live cell) imaging, including z-stacks, movies and time-lapse experiments, photobleaching approaches such as Fluorescence Recovery After Photobleaching (FRAP) and related techniques and also Fluorescence Correlation Spectroscopy (FCS). The system is well suited for fluorescence lifetime measurements, e.g. in FRET applications and, as a unique feature, for multiparameter fluorescence detection in confocal images.