The PerkinElmer Operetta CLS is an automated microscopy system for high-content analysis (HCA). It provides rapid image acquisition through spinning disk technology and high sample throughput through the use of 96-well or 384-well microplates. Fluorescence and transmitted light images can be acquired in confocal and non-confocal modes.
Installed objectives (all air):
- 5x / NA 0.16
- 20x / NA 0.4
- 40x / NA 0.75
Light path/ optics:
4x LED light source, 5x emission filter, Spinning disk optics. 1x top-down-LED (740 nm, for brightfield and digital phase contrast images).
Excitation windows available:
- 370 nm (355-385 nm)
- 475 nm (460-490 nm)
- 550 nm (530-560 nm)
- 630 nm (615-645 nm)
Detection windows available:
- 430-500 nm (blue)
- 500-550 nm (green)
- 515-580 nm (yellow)
- 570-650 nm (red)
- 665-760 nm (far-red)
Camera: 4.7 Megapixel sCMOS sensor, image dimension of 2160x2160 px.
Incubation: Heating is available (37-42 °C; system temperature of approx. 25 °C). CO2 controller available (1-10 %). No humidity control.
In addition, a slide holder is available to acquire images of fixed tissues.